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Search Toggle search field. Toggle search field Toggle search field. Brings extensive automotive industry expertise that includes helping clients with transformation programs, strategy and organizational initiatives and digital manufacturing projects.
Key contributor to our electric mobility and lightweight research projects. Recent projects have involved: Designing and supporting turnaround programs for the product divisions of automotive suppliers.
As part of this work, he identified EBIT improvement levers across the value chain i. The program featured different work-streams to reduce operational costs and accelerate decision-making processes.
Introducing a holistic product cost management system at a passenger car OEM. The study included a detailed assessment of the processes, tools and methods for target costing, and defined the future system for proactive product cost management for new car development.
Designing and supporting a product development efficiency improvement program at an automotive supplier.
Defining a go-to-market strategy for an Asian commercial vehicle company for medium-duty trucks. A new type of a strictly anaerobic, mesophilic bacterium was enriched and isolated with gluconate as sole substrate from a methanogenic sludge collected from a biogas reactor.
A broad variety of utilized substrates include gluconate, glucose, fructose, maltose, sucrose, lactose, galactose, melezitose, melibiose, mannitol, erythritol, glycerol and esculin.
Products of gluconate fermentation were ethanol, acetate, formate, H2 and CO2. Sulfate or nitrate did not serve as electron acceptor. The closest cultured relatives are Clostridium herbivorans Pelobacter carbinolicus and P.
Cocultures with Methanospirillum hungatei served as model systems for the elucidation of syntrophic ethanol oxidation previously done with the lost "Methanobacillus omelianskii" coculture.
Two different acetaldehyde-oxidizing activities were found: Tungsten limitation resulted in slower growth and the expression of a molybdenum-dependent isoenzyme.
Putative comproportionating hydrogenases and formate dehydrogenase were expressed constitutively and are probably involved in interspecies electron transfer.
In ethanol-grown cocultures, the maximum hydrogen partial pressure was about 1, Pa 1 mM while 2 mM formate was produced.
Hydrogen and formate formation and degradation further proved that both carriers contributed to interspecies electron transfer.
The maximum Gibbs free energy that the Pelobacter species could exploit during growth on ethanol was to kJ per mol ethanol.
Both species could be cultivated axenically on acetaldehyde, yielding energy from its disproportionation to ethanol and acetate.
Syntrophic cocultures grown on acetoin revealed a two-phase degradation: Protein expression and activity patterns of both Pelobacter spp. The early assumption that acetaldehyde is a central intermediate in Pelobacter metabolism was now proven biochemically.
In syntrophic conversion of butyrate to methane and CO2, butyrate is oxidized to acetate by secondary fermenting bacteria such as Syntrophomonas wolfei in close cooperation with methanogenic partner organisms, e.
Formate dehydrogenase-activity staining of dodecylmaltoside-solubilised crude extract from butyrate-grown S.
Amino-acid sequence alignment in an attempt to predict the ion specificity of the ATP synthase of S. Identifications obtained by peptide fingerprinting-mass spectrometry for the protein bands excised from activity-stained gel strips.
Identifications obtained by peptide fingerprinting-mass spectrometry for protein bands excised from an activity-stained gel strip after separation of dodecylmaltoside-solubilised crude extract.
Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum.
Background Thermacetogenium phaeum is a thermophilic strictly anaerobic bacterium oxidizing acetate to CO2 in syntrophic association with a methanogenic partner.
It can also grow in pure culture, e. The key enzymes of homoacetate fermentation Wood-Ljungdahl pathway are used both in acetate oxidation and acetate formation.
The obvious reversibility of this pathway in this organism is of specific interest since syntrophic acetate oxidation operates close to the energetic limitations of microbial life.
Results The genome of Th. A complete gene set for a phage was found in the genome, and indications of phage action could also be observed in culture.
The genome contained all genes required for CO2 reduction through the Wood-Ljungdahl pathway, including two formyl tetrahydrofolate ligases, three carbon monoxide dehydrogenases, one formate hydrogenlyase complex, three further formate dehydrogenases, and three further hydrogenases.
The bacterium contains a menaquinone MQ No indications of cytochromes or Rnf complexes could be found in the genome. Conclusions The information obtained from the genome sequence indicates that Th.
The specific enzyme outfit of Th. List of putative prophage genes. Effects of hydrogen and acetate on benzene mineralisation under sulphate-reducing conditions.
Syntrophic mineralisation of benzene, as recently proposed for a sulphate-reducing enrichment culture, was tested in product inhibition experiments with acetate and hydrogen, both putative intermediates of anaerobic benzene fermentation.
Using [ 13 C 6 ]-benzene enabled tracking the inhibition of benzene mineralisation sensitively by analysis of 13 CO 2.
In noninhibited cultures, hydrogen was detected at partial pressures of 2. In cultures spiked with higher amounts of hydrogen, benzene mineralisation did not restart after hydrogen consumption, possibly due to the toxic effects of the sulphide produced.
An inhibitory effect was also observed when acetate was added to the cultures 0. Benzene mineralisation resumed after acetate was degraded to concentrations found in noninhibited cultures, indicating that acetate is another key intermediate in anaerobic benzene mineralisation.
Although benzene mineralisation by a single sulphate reducer cannot be ruled out, our results strongly point to an involvement of syntrophic interactions in the process.
Syntrophy in Methanogenic Degradation. Sep Endo symbiotic Methanogenic Archaea. This chapter deals with microbial communities of bacteria and archaea that closely cooperate in methanogenic degradation and perform metabolic functions in this community that neither one of them could carry out alone.
The methanogenic degradation of fatty acids, alcohols, most aromatic compounds, amino acids, and others is performed in partnership between fermenting bacteria and methanogenic archaea.
The energy available in these processes is very small, attributing only fractions of an ATP unit per reaction run to every partner.
The biochemical strategies taken include in most cases reactions of substrate-level phosphorylation combined with various kinds of reversed electron transport systems in which part of the gained ATP is reinvested into thermodynamically unfavourable electron transport processes.
Altogether, these systems represent fascinating examples of energy efficiency at the lowermost energy level that allows microbial life.
Syntrophic butyrate and propionate oxidation processes: Environ Microbiol Rep 2: In anoxic environments such as swamps, rice fields and sludge digestors, syntrophic microbial communities are important for decomposition of organic matter to CO2 and CH4.
The most difficult step is the fermentative degradation of short-chain fatty acids such as propionate and butyrate. Conversion of these metabolites to acetate, CO2 , formate and hydrogen is endergonic under standard conditions and occurs only if methanogens keep the concentrations of these intermediate products low.
Butyrate and propionate degradation pathways include oxidation steps of comparably high redox potential, i. Although investigated for several decades, the biochemistry of these reactions is still not completely understood.
Genome analysis of the butyrate-oxidizing Syntrophomonas wolfei and Syntrophus aciditrophicus and of the propionate-oxidizing Syntrophobacter fumaroxidans and Pelotomaculum thermopropionicum reveals the presence of energy-transforming protein complexes.
Recent studies indicated that S. Reversed electron transport in syntrophic degradation of glucose, butyrate and ethanol. Methanogenic oxidation of butyrate to acetate requires a tight cooperation between the syntrophically fermenting Syntrophomonas wolfei and the methanogen Methanospirillum hungatei, and a reversed electron transport system in S.
The metabolic activity of butyrate-oxidizing S. In cell extracts of S. Recovered proteins were identified by peptide mass fingerprinting, which indicated the presence of an NADH: The results suggest that syntrophic oxidation of butyrate in S.
Dominant sugar utilizers in sediment of Lake Constance depend on syntrophic cooperation with methanogenic partner organisms. Six strains of novel bacteria were isolated from profundal sediment of Lake Constance, a deep freshwater lake in Germany, by direct dilution of the sediment in mineral agar medium containing a background lawn of the hydrogen-scavenging Methanospirillum hungatei as a syntrophic partner.
The numbers of colony-forming units obtained after incubation for more than 2 months were in the same range as those of total bacterial counts determined by DAPI staining up to 10 8 cells per millilitre suggesting that these organisms were dominant members of the community.
Identical dilution series in the absence of methanogenic partners yielded numbers that were lower by two to three orders of magnitude.